Odontoblasts and/or dental pulp cells are responsible for tooth repair as well as dentin formation. Adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether Pam3 modulates adhesion and migration of a murine odontoblast-like cell line, MDPC-23 cell and TLR2 signaling is engaged in this process. TLR2 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type Ⅰ collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, JNK, p38, and ERK was examined by Western blot analysis. TLR2 was functionally expressed in MDPC-23 cells. Pam3CSK treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR2 using its antibody restored Pam3CSK-induced adhesion and migration of MDPC-23 cells. These findings indicate that Pam3CSK, an immune activator from gram negative bacteria, can promote adhesion and migration ability of MDPC-23 cells via TLR2.

Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 세포배양 및 시약
  2. Reverse transcription-polymerase chainreaction (RT-PCR)
  3. MTT 분석
  4. Western blot 분석
  5. Cell Adhesion 분석
  6. 세포 이동 분석
  7. 자료 분석
 Ⅲ. 결과
  1. MDPC-23 세포에서 TLR2 발현 및 NF-κB와 MAPK신호 활성화
  2. TLR2 agonist가 MDPC-23 세포 성장에 미치는 영향
  3. TLR2 agonist가 MDPC-23 세포 부착에 미치는 영향
  4. TLR agonist가 MDPC-23 세포 이동에 미치는 영향
 Ⅳ. 고찰
 V. 참고문헌

• 안미영(원광대학교 치과대학 대전치과병원 구강병리과) | Mee Young Ahn
• 신동은(원광대학교 치과대학 대전치과병원 구강병리과) | Dong Eun Shin
• 권성민(원광대학교 치과대학 대전치과병원 구강병리과) | Seong Min Kwon
• 안지위(원광대학교 치과대학 대전치과병원 구강병리과) | Ji Wee Ahn
• 이준(원광대학교 치과대학 대전치과병원 구강악안면외과) | Jun Lee
• 윤정훈(원광대학교 치과대학 대전치과병원 구강병리과) | Jung Hoon Yoon correspondence