Various type of composite beams have been developed to utilize advantages of reinforced concrete and steel structural system with making up for their weaknesses. In this study, prestressed composite beams with discontinuous corrugated webs are presented, in which discontinuous web plates improve the introduction efficiency of prestress by accordion effect and enhance composite action between steel beam and concrete compared to typical plane webs. Two prestressed composite beam with discontinuous corrugated webs, four prestressed composite beam with trapezoidal corrugated webs, and one corrugated webbed composite beam without prestress were fabricated and tested to evaluate their serviceability, ductility and flexural strength.