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Molecular biological characteristics and biosafety assessment for drought-tolerant transgenic rice (CaMsrB2)
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/294315
Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by various agricultural biotechnological companies. Commercialization of GM crops will be required the assessment of risk associated with the release of GM crops. In this study, we carried out to provide the molecular characterization of introduced T-DNA in transgenic rice T4 ~ T6 generation lines harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (CaMsrB2). We identified the structure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice lines. The transformation vector was consisted of right border, a drought-tolerant CaMsrB2 gene unit, a selectable marker herbicide resistance unit, and left border in a sequential order. Based on the adaptor-ligation PCR and whole genome sequence database, we confirmed that T-DNA was introduced at the position of 41,737,284 bp of chromosome No. 1. From the generational stability study, T-DNAs were stably inherited through the T4 to T6 generations, and also stable expression of bar gene from T-DNA was confirmed. These results will be filed to biosafety assessment document of CaMsrB2 rice.
