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Towards high-throughput marker-assisted backcrossing system

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  • URLhttps://db.koreascholar.com/Article/Detail/294337
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한국육종학회 (The Korean Breeding Society)
초록

The main objectives of IRRI’s variety development should meet the needs of customers/farmers from diverse rice sectors in each target region. The dynamic market change asks rapid variety development with highly valued QTLs/genes. Molecular breeding implemented through the efficient crossing, high throughput genotyping and rapid generation advancement will provide packages to breeders to develop new varieties quickly and more economically. The more efficient and cost-effective marker-assisted backcrossing service will provide the more opportunity for the success in molecular breeding platform. To make MABC system more successful, the development of molecular marker system for the high-throughput SNP genotyping is must. Currently Genotyping Service Lab (GSL) of IRRI provides high-throughput SNP genotyping service using BeadXpress and Fluidigm system. Meanwhile, the linked SNP markers for the specific traits are being developed. For abiotic stress tolerances, the markers for submergence, drought, heat, anaerobic germination, salinity, and phosphorus deficiency for Fluidigm system are being developed and tested in variety diversity panel and segregating populations. In MABC, due to the high number of crossings, the labor- and space-saving crossing system is being developed. As a result of an integrated MABC platform will speed up the development of pre-breeding line which are containing single or multiple QTLs/genes.

저자
  • Chenie Zamora(Plant Breeding, Genetics, and Biotechnoloy Division, The International Rice Research Institute, The Philippines) Corresponding Author
  • Katreena Titong(Plant Breeding, Genetics, and Biotechnoloy Division, The International Rice Research Institute, The Philippines)
  • Michael J. Thomson(Plant Breeding, Genetics, and Biotechnoloy Division, The International Rice Research Institute, The Philippines)
  • Joong Hyoun Chin(Plant Breeding, Genetics, and Biotechnoloy Division, The International Rice Research Institute, The Philippines)