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Studies of R2R3-OsMYB4P transcription factor in phosphate starvation signaling
The molecular processing of upstream regulation of Pi response genes during Pi starvation remains inadequately understood. Several transcription factor have been studied that appear to regulate subsets of the responses to Pi stress either positively or negatively. MYB genes are responsive to one or multiple type of hormone and stress treatments. In this study, cDNA of the MYB have been cloned, and we generated Rice overexpressing plants for characterization of these genes. OsMYB gene function focused on phosphate conditions with rice and Arabidopsis transgenic plants. We selected 30 - T1 transgenic lines from T0 transgenic rices. those are shown high Pi content. The Pi contents of shoots part of transgenic plants were shown 10~20% increased Pi contents than WT, whereas roots have 30% increased Pi contents. As a result, OsMYB genes affect Pi uptake in plants. To investigate interactions between MYB proteins and phosphate signaling related genes. We demonstrate that Myb-binding sites (MBSs) exist in putative promoter of OsPT transporter by analysis of bioinformatics, and its bind specific MYB transcription factor. OsMYB expression is induced by low Pi and Pi deficiency, and its overexpression plants are shown morphological phenotype in Pi stress.
