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Isolation and characterization of novel gene and microsocopic analysis of a rice mutant with narrow leaves.
Rice is not only a model plant of monocots but also one of the most important crops all over the world. Despite the importance of leaf shape for achieving effective plant architecture for photosynthesis, little is known about the genetic mechanisms that determine leaf morphological characteristics. Explanation of the genetic basis of the control of leaf shape could be of use in the manipulation of crop traits, leading to increased crop production. Many mutants related to leaf morphology have been identified and classified according to their function in determining leaf morphology. search on the genetics of leaf development has used mutagensis to create loss-of-function mutations that change leaf shape. To understand the molecular mechanism of leaf morphogenesis, we identified a rice mutant gene, which was characterized by a phenotype of narrow leaves. While the mutation resulted in reduced leaf width, no significant morphological changes at the cellular level in leaves were observed, except in bulliform cells. The gene locus guess that it encodes a adenosine kinase, which displays sequence homology with ribokinase pfkB like superfamily. To test function of gene, we cloned gene which have 1140 nucleotides and 379 amino acids. This gene was transcribed in various tissues and was mainly expressed in panicles and leaves. NAL7, NAL1 and SLL1 were found to be downregulated, whereas OsAGO7 and NRL1 were upregulated in the mutant. These findings suggested that there might be a functional association between these genes in regulating leaf development.
