The most important factor in breeding program is to obtain the value-added genetic line. Generally, breeders develop genetic sources using several methods such as segregation-breeding, cross-breeding, backcross-breeding, mutation induction, tissue culture and so on. Here, we present one classical way but very valuable method called cell fusion or protoplast fusion to create genetic sources for the breeding practice. The method we developed was the asymmetric somatic-hybridization of protoplast isolated from carrots. This is rather to transfer the nucleus from the high quality F1 hybrid to other mediocre line to produce a new carrot line. Since the breeding a carrot line for higher quality and purity takes a long time, therefore this nuclear transfer technology is very beneficial to generate a new line that could be useful to breed elite varieties. We had obtained around 200 fused carrots (cybrids), 12 cybrids were self pollinated and produced seeds. Selected progenies have been evaluated for horticultural characteristics and we have found new genetic lines that show better phenotypes.

• Min Jung(Biotechnology Institute, Nongwoo Bio Co.)
• Da-Hae Son(Biotechnology Institute, Nongwoo Bio Co.)
• Ji-Young Hyun(Biotechnology Institute, Nongwoo Bio Co.)
• Young-Woo Liu(Breeding Institute, Nongwoo Bio Co.)
• Sih-Woo Lee(Breeding Institute, Nongwoo Bio Co.)
• Chee-Hark Harn(Biotechnology Institute, Nongwoo Bio Co.) Corresponding Author