Soybeans [Glycine max (L.) Merr] are an important source of nutrients including protein, oils and various useful secondary metabolites. Phosphatidylcholine (PC) component serves as a nerve cell membrane material and a choline supplier, so it may improve memory function in subjects suffering from memory impairment and dementia. Content of PC component in soybean seed may depend on genotype and environment. Genotype with high PC content is valuable in breeding project. Fifty-seven soybean genotypes were cultivated at first year. After harvesting, PC contents were analyzed. Content (mg/kg) of PC component was from 7.02 to 19.55. At second year, 111 genotypes including 57 genotype used at first year were cultivated. After harvesting, PC contents were analyzed. Content (mg/g) of PC component was from 0.061 to 12.324.