Soybean is an excellent source of protein for human and animal feed. But, nutritional quality is compromised by a low content of the sulfur amino acid, methionine. The purpose of this research is to screen the genotype with high methionine content. Forty genotypes including current cultivars, breeding lines, and germplasms were evaluated in the for two years. After harvest, random seeds of each genotypes were used to check methionine content by HPLC method. Methionine contents ranged from 1.9 - 5.0 (mg/g) for first year to 2.4 - 4.3 (mg/g) for second year. At first year, three genotypes [76F7-4(1), 86F8-2(1), and 70F7-1] had high content of methionine. At second year, three genotypes [Jinnon#1, daalkong, 86F8-2(1)] had high content of methionine. Genotype, 86F8-2(1) had high content of methionine at both years. Also, effect of environment on methionine content was observed. However, methionine content is a very different from genotypes.