This study was conducted to investigate plant body temperature response of soybean (Glycine max) to saline stress. Two-weeks-old seedlings of soybean in V1 growth stage were treated with 0, 10, 20, 40, 80 and 160 mM of NaCl for salt stress. Thermal images acquired using Flir T-420 (US) were obtained at 4 days after treatment. Soybean leaf temperature increased with increasing NaCl concentration, resulting in significant positive correlation between soybean leaf temperature and stress intensity (P < 0.01). Leaf temperature of soybean was significantly different at 160 mM of NaCl, where no visual symptom was observed. Therefore, soybean leaf temperature can be used for evaluating the response of soybean to salt stress as a non-destructive and phenomic parameter. Non-destructive diagnosis of soybean leaf temperature may be a key parameter in a high throughput screening (HTS) system in breeding program for salt stress tolerance soybean cultivars.