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Genetic mapping and QTL analyses for eating quality-related traits in F2:3 population derived from waxy corn x sweet corn
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/297897
In this study, we were conducted the construction of the framework map using SSR markers in the F2 population derived from a cross between waxy corn inbred line (02S6140) and sweet corn inbred line (KSS22), and also identifying of QTLs associated with eating quality traits by employing genetic linkage map of F2:3 population. The linkage map was constructed using 295 SSR markers on the 158 F2 individuals derived from a cross of 02S6140 and KSS22. The map comprised a total genomic length of 2,626.5cM in ten linkage groups and an average distance between markers of 8.9cM. Chi-square test revealed that 254 markers (86.1%) associating with all ten chromosomes exhibited a segregation of 1:2:1 Mendelian ratio. A total of 10 QTLs each for pericarp thickness (PER), amylose content (AMY), dextrose content (DEX), and sucrose content (SUC) were detected in the 158 F2 families. The number of QTL per each trait was ranged from 2 to 4, and also phenotypic variance was ranged from 4.26 to 30.71%. For PER, 4 QTLs were found to be controlled by four genomic regions at locations chromosomes 4, 5, 8, and 9 contributing 10.43, 6.71, 6.74, and 7.79% of phenotypic variance, respectively. While 2 QTLs for AMY, DEX, SUC traits, were found to be controlled by two genomic regions at locations chromosomes 4, 6, 8, and 9 contributing between 4.26 and 30.71% of phenotypic variance, respectively. Among them, 4 QTLs, such as qAMY4 (10.43%), qAMY9 (19.33%), qDEX4 (21.31%), and qSUC4 (30.71%), may be considered as a major QTLs, while the remaining six QTLs might be regarded as minor QTLs. In our study, qAMY9 for amylase content was detected on chromosome 9 in marker intervals phi027-umc1634, which was the same locus as encoding wx1 gene. Thus qAMY9 may be thought very useful molecular marker for selecting amylase content trait. The other QTLs may be thought very useful molecular marker for eating quality traits. The resulting genetic map will be useful in dissection of quantitative traits and the identification of superior QTLs from the waxy hybrid corn, and also this study may provide valuable information for the further identification and characterization of genes responsible for eating quality-related traits in waxy corn and sweet corn.
