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Functional analysis of a novel gene encoding DnaJ-like protein from soybean

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  • URLhttps://db.koreascholar.com/Article/Detail/297904
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한국육종학회 (The Korean Breeding Society)
초록

We have isolated wound-inducible genes from soybean using suppression subtractive hybridization (SSH) method and were able to obtain the full-length clone of GmDjp1 gene encoding DnaJ-like protein. The full-length cDNA of GmDjp1 is 689 bp with an open reading frame (ORF) consisting of 163 amino acid (aa). Genomic southern blot confirmed that soybean genome has two copies of GmDjp1 gene. Northern blot analysis showed that the RNA expression of GmDjp1 gene is specifically induced by heat, NaCl, wounding and drought stresses. It was demonstrated that GmDjp1-GFP was targeted to the nucleus in tobacco cell. GmDjp1 overexpression plants showed more susceptible to salt and heat stress compared to WT. RNA expression level of Hsp18.2 and Hsp25.3-P was lower than that of WT during recovery after heat hock in plants. This indicates that GmDjp1 may play a negative regulator to stress responses in plants.

저자
  • Hyun-A So(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Eunsook Chung(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Kyoung-Mee Kim(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Selvam Ayarpadikannan(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Kenneth Ryan Schraufnagle(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Kim Hyo Young(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Jae-Sung Kwak(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Hai Yang Yu(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Jai-Heon Lee(BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University) Corresponding Author