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User friendly molecular breeding platform by analyzing soybean genomes

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  • URLhttps://db.koreascholar.com/Article/Detail/297967
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한국육종학회 (The Korean Breeding Society)
초록

Resequencing data is actively used for searching QTL or analyzing genetic diversity in the crops. However, the complexity of genome, caused by genome duplication, limits the utility of genome-wide association studies and linkage analyses to identify genes that regulate agronomically valuable traits. Here, we propose a comparative genomics approach based on core or common variation-based recombination blocks (CRB) using single nucleotide variation (SNV) density information. We found that the soybean genomes are assembled with long and distinct CRBs as large as 10Mb. CRB-based comparative genomics enabled us to accurately identify recombination blocks at the whole-chromosome level. We identified the Ih locus that determines the yellow hilum color in soybeans using CRB-based mapping with representative indel markers. These results suggest that the CRB-based comparison method is a promising platform for molecular breeding and map-based cloning.

저자
  • Yul Ho Kim(National Institute of Crop Science, Rural Development Administration) Corresponding Author
  • Tae-Young Hwang(National Institute of Crop Science, Rural Development Administration)
  • Hyang Mi Park(National Institute of Crop Science, Rural Development Administration)
  • Seuk Ki Lee(National Institute of Crop Science, Rural Development Administration)
  • Man Soo Choi(National Institute of Crop Science, Rural Development Administration)
  • Kwang Ho Jeong(National Institute of Crop Science, Rural Development Administration)
  • Min Jung Seo(National Institute of Crop Science, Rural Development Administration)
  • Hong Tai Yun(National Institute of Crop Science, Rural Development Administration)
  • Sun Lim Kim(National Institute of Crop Science, Rural Development Administration)
  • Young-Up Kwon(National Institute of Crop Science, Rural Development Administration)
  • Ik-Young Choi(National Instrumentation Center for Environmental Management, College of Agriculture and Life Science, Seoul National University)
  • Ho-Sung Yoon(Department of Biology, Kyungpook National University)
  • Suk-Ha Lee(Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Jong Bhak(Theragen Bio Institute, TheragenEtex, Personal Genomics Institute, Genome Research Foundation)
  • Sunghoon Lee(Theragen Bio Institute, TheragenEtex, Personal Genomics Institute, Genome Research Foundation)