To produce abiotic stress resistant transgenic cucumber, the cotyledonary node explants of cucumber (c.v. Eunsung) were inoculated with A. tumefaciens strain EHA105 containing the binary vector (pPZP211) carrying Nit gene. The 491 explants inoculated with bacterium solution for 30 min were maintained on 50 mg/L paromomycin contained shoot induction (SI) medium for first 2 weeks and then subcultured on 100 mg/L paromomycin to obtain transgenic adventitious shoots for 4 x 14 days. So far, 5 plant were selected, and then acclimated in soil. Of them, 3 transgenic plants with Nit gene were confirmed by Southern blot analysis.

• Hyun A Jang(Korea Research Institute of Bioscience and Biotechnology, Department of Biology, Chungnam National University)
• Ga Min Lim(Department of Medicinal Plant Resources, Nambu University, Medicinal Plant Transformation Center, Nambu University)
• Hyun A Kim(Korea Research Institute of Bioscience and Biotechnology, Biosystems and Bioengineering Program, University of Science and Technology (UST))
• Youn-il Park(Department of Biology, Chungnam National University)
• Suk Yoon Kwon(Korea Research Institute of Bioscience and Biotechnology, Biosystems and Bioengineering Program, University of Science and Technology (UST)) Corresponding Author
• Pil Son Choi(Department of Medicinal Plant Resources, Nambu University, Medicinal Plant Transformation Center, Nambu University) Corresponding Author