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Studies on Phylogenetic Relationships and Mycelial Growth Characteristics in Hypsizygus marmoreus (Peck) Bigelow Strains
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298007
The purpose of this study was to develop the best mushroom cultivation conditions and the combination of mushroom culture media in order for mushroom producers and consumers. To reach this target, we first investigated the genetic relationship and developed suitable conditions of mycelial growth in Hypsizygus marmoreus strains. One superior strain of H. marmoreus was selected from 124 strains using bag culture. One hundred and twenty four strains were genetically classified into four main groups using two Universal Rice primers, URP2R and URP17R. The studies on the effects of different temperature (17, 21, 25, 29, 33℃) showed that 25℃ is the best temperature for mycelial growth for almost all strains while at 33℃ most of mycelium stop growth. Finally, ten strains were selected according to the groups identified by their temperature requirements. The length of mycelial growth in PDA, MCM, GPYM, MEA and MYP were longer than those in Czapek Dox. The selected ten strains of H. marmoreus showed heavier dry weight of mycelia at pH 3.0∼7.0 than any other pH. Although it was not show distinct requirement of carbon and nitrogen sources for vegetative growth according to strains, mainly the mycelial growth of the ten selected strains were observed at media including xylan and yeast extract, peptone, tryptone, respectively. Moreover, higher C/N ratio was observed in higher dry weight of mycelia.
