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Genome-wide identification and characterization of miniature inverted- repeat transposable elements in Brassica rapa

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  • URLhttps://db.koreascholar.com/Article/Detail/298032
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한국육종학회 (The Korean Breeding Society)
초록

Miniature inverted- repeat transposable elements are expected to play vital role in evolution of genes and genome of major eukaryotic organisms. However, there have been little reports on MITEs in B. rapa, a polyploidy model genome. We identified 13 novel MITE families in B. rapa genome by computational approach. Out of 13 MITEs families three, eight and two were classified under stowaway-like, tourist-like and hAT super families based on their unique structural characteristics. We characterized the members of 13 MITE families from the available 256 Mbp from whole genome draft sequences of B. rapa. We found ech MITE has high copiy number ranges from 14 to 977 which are distributed randomly along all the chromosomes. We also found more than 40% of the MITE members were associated with genes and gene rich regions. Furthermore, the polymorphism due to insertion and non-insertion of MITEs analysis suggest that MITEs are active in the genome. As, such the newly identified MITEs will provide a foundation for the further analysis of roles of MITEs in gene and genome evolution.

저자
  • Perumal Sampath(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Sang-Choon Lee(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Jee Young Park(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Jonghoon Lee(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Nur Kholilatul Izzah(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Shailendra Karki(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Haejoon Joo(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University)
  • Tae-Jin Yang(Dept. of Plant Science, Plant Genomics and Breeding Institute for Agriculture and Life Sciences, Seoul National University) Corresponding Author