The Korean people consume a lot of hot peppers. Despite its popularity and importance, genetic mechanism determining the content of capsaicinoid has not been understood yet. Since the inheritance of capsaicinoid content is thought to be controlled by quantitative trait loci (QTLs), a well-constructed genetic population and well-organized approach method to study genetic inheritance are required. Here we show the scheme to find the QTLs controlling capsaicinoid content using a recombinant inbred lines (RILs) obtained from a cross between C. annuum, Perennial and C. annuum, Dempsey. We measured capsaicinoid contents of each line for QTL analysis. In addition, total 24 qualitative and quantitative traits of RILs were phenotyped. Phenotyping data can be used to construct another QTL map controlling agricultural trait. An ultra high density linkage map constructed by sequencing RILs will used for QTL nalaysis. We are expecting that this study will be useful to breed high-quality and pungent pepper cultivars by molecular breeding method.

• Koeun Han(Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
• Hee-Jin Jeong(Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
• Jin-Kyung Kwon(Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
• Byoung-Cheorl Kang(Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University) Corresponding Author
• Yong-Jik Lee(Hana Seed company)