논문 상세보기
Construction and characterization of Squash and Oriental melon BAC libraies
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298050
Cucumis melo L. is an economically important crop. Genetically, Cucumis melo and Cucurbita maxima belong to Cucurbitaceae family, however, genetic characteristics such as genome size and stress tolerance, are different. To provide genomic resources for the Cucurbitaceae crop improvement, we constructed two bacterial artificial chromosome (BAC) libraries for oriental melon (Gotgam chamoe) and squash (Cucurbita Maxima), named ‘Gotgamchamoe BACH1’ and ‘Maxma BACH1’, respectively. Three hundred eighty four BACs were randomly selected and analyzed by Not I restriction enzyme digestion in order to evaluate the libraries in terms of insert size, empty clone ratio. Gotgamchamoe BACH1 was consisted of 92,160 BAC clones with an average insert size of 116kb ranging from 8 to 252kb. Maxima BACH1 was comprised 158,380 clones with an 116Kb of an average insert size ranging 8 to 305Kb. The both libraries were represented 20 haploid genome equivalents based on the reported genome sizes of 500Mb and 900Mb for Gotgamchamoe and maxima. The empty ratios of both libraries were 1.04 and 1.50% for Gotgamchamoe and maxima, respectively. These high quality BAC resources will provide a useful platform for genomic research and crop improvement for Cucurbitacea crops.
