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Independent Inheritance between ti gene and y9 gene in soybean
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298143
Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. Soybean Kunitz trypsin inhibitor protein has been proposed as one of the major antinutritional factor. The absence of Kunitz trypsin inhibitor protein in mature seed is inherited as a recessive allele designated ti. The objective of this research was to confirm independent inheritance between ti gene and y9 gene. The F1 seeds from Gaechuck#1 (Kunitz trypsin inhibitor protein absent, normal leaf type) x C-142 (Kunitz trypsin inhibitor protein present, leaf chlorophyll -deficient) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of Kunitz trypsin inhibitor protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.
