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Independent Inheritance between cgy1 gene and y9 gene in soybean
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298144
Soybean [Glycine max (L.) Merr.] proteins are widely used for human and animal feed in the world. Glycinin (11S globulin) and β-conglycinin (7S globulin) account for storage protein from 70% to 80% in soybean seed. 7S globulin protein exhibits poorer nutritional and food processing properties. β-conglycinin is composed of α’, α, and β-subunits. α′-subunit of 7S globulin are main antinutritional factors in soybean seed. The absence of α′-subunit were controlled by single recessive alleles, cgy1. Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic studies in soybean. So far, 19 single recessive gene yellow leaf mutants and one duplicate recessive gene mutant have been described. The y9 type found in T135 is yellow at emergence, becoming greenish-yellow by maturity. The objective of this research was to confirm the linkage or independent assortment between cgy1 gene and y9 gene. The F1 seeds from C-142 (α′-subunit of 7S globulin present, leaf chlorophyll -deficient: Cgy1Cgy1y9y9 genotype) x PI line (α′-subunit of 7S globulin absent, normal leaf type: cgy1cgy1Y9Y9 genotype) were obtained. F1 seeds obtained were planted in a greenhouse and F1 hybridity was checked on morphological traits. All F2 seeds were planted at field in May 2012. Leaf chlorophyll-deficient trait of F2 individual plants will be recorded at growth stage from field. Presence and absence of α′-subunit of 7S globulin protein will be checked by SDS-PAGE based on each F₂single plant. Chi-square analysis was used to test the goodness-of-fit of observed ratios with expected ratios for independent assortment or linkage.
