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Genetic structure of Korea Brassica rapa core collection based on Mariner transposon display
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/298152
We have identified ATTIRTA1 transposon, a kind of mariner-type DNA transposon from Brassica rapa genome. A total of 811 inverted-terminal repeat, ITR consisting of the both terminal on ATTIRTA1 transposon were found from B. rapa v1.1 sequence. Among them 616 ITR were paired by two in each transposon, indicating three quarters of the transposon exists in original form. Around 10 percentage of the transposon, 82 ITR was located in gene, expecially only in intron. Using these ATTRRTA1, we developed a display system modified from AFLP technique and applied for this system to analyze genetic diversity of Korea Brassica rapa core collection. The collection includes 220 accessions representing the different morphotypes and geographical origin. The analysis of population structure revealed five subgroups and the clustering patterns matched well with their morphological traits. ATTIRTA1 transposon display seems useful marker system for studying genetic relationships. Presently we have profiled the components and contents of glucosinolate in the core collection to analyze genome wide association. This collection will be helpful to identify agriculturally desirable traits from other supspecies.
