Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen (10 μg/ml) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary.

서 론
 재료 및 방법
  1. 실험 재료
  2. 난소표피세포 분리 및 배양
  3. 마이크로어레이 분석
  4. 정량적 역전사중합효소중합반응(qRT-PCR)
  5. 단백질 분석(Western Blot Analysis)
  6. 통계학적 분석 및 실험 성적의 처리
 결 과
  1. 난포낭종에서 변화되는 이온 및 수송체 관련 유전자 선별
  2. 난포낭종에서 증가하는 AQP7의 발현 검증
  3. 에스트로겐에 의한 AQP7의 단백질 발현 증가
 고 찰
 결 론
 참 고 문 헌

• 김창운(삼성창원병원 산부인과) | Chang-Woon Kim
• 한신규(부산대학교 분자생물학과) | Sunkyu Han
• 최창용(국립축산과학원 가축유전자원센터) | Changyong Choe Correspondence