The peel of Citrus sunki exhibits multiple biological activities such as anti-oxidant, anti-inflammation and anti-obesity, but little is known about neurodegeneration-related activities. In this study, we investigated the protective effect of ethanolic extract from both immature and mature Citrus sunki peel on neuronal cell death. Treatment of the neuroblastoma cell line SH-SY5Y with MPP+, an inducer of Parkinson disease model, increased cell death in a dose dependent manner. Increased levels of active caspase-3 and cleaved PARP were detected. Treatment with immature Citrus sunki peel extract significantly reduced MPP+-induced neurotoxicity. Cytoprotection with immature Citrus sunki peel extract was associated with a decrease in caspase-3 activation and PARP cleavage. In contrast, mature Citrus sunki peel extract had no significant effects. These data suggest that immature Citrus sunki peel extract may exert anti-apoptotic effect through the inhibition of caspase-3 signaling pathway on MPP+-induced neuronal cell death.

서 언
 재료 및 방법
  1. 과피 추출물 제조
  2. 세포배양
  3. 세포 생존율 측정
  4. 세포 독성 평가
  5. Western blot
  6. 통계 처리
 결과 및 고찰
  1. MPP+의 신경세포 사멸 효과
  2. 미성숙 진귤 과피 추출물의 신경세포 보호 효과
 감사의 글
 REFERENCES

• 고운철(제주대학교 생물학과) | Woon Chul Ko
• 이선령(제주대학교 생물학과) | Sun Ryung Lee Corresponding author