Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (≥ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose- 1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

MATERIALS AND METHODS
  Plant growth and preparation of P. grandiflorum extracts
  Extraction of proteins
  Two-dimensional gel electrophoresis and image analysis
  In-gel digestion
  Mass spectrometry (MALDI-TOF-TOF/MS) analysis
  Bioinformatics
 RESULTS AND DISCUSSION
  Separation of proteins by 2-DE
  Functional categorization of the identified proteins
  Identified proteins and its’ implication from variousorgans of Platycodon grandiflorum
 CONCLUSION
 ACKNOWLEDGEMENT
 REFERENCES

• Hye-Rim Kim(Dept. of Crop Science, Chungbuk National University)
• Soo-Jeong Kwon(Dept. of Food Nutrition and Cookery, Woosong College)
• Swapan Kumar Roy(Dept. of Crop Science, Chungbuk National University)
• Seong-Woo Cho(Rice Research Division, National Institute of Crop Science RDA)
• Hag-Hyun Kim(Dept. of Food Nutrition and Cookery, Woosong College)
• Kab-Yeon Cho(Dept. of Food Nutrition and Cookery, Woosong College)
• Hee-Ock Boo(WellPhyto Co. Ltd., BI Center)
• Sun-Hee Woo(Dept. of Crop Science, Chungbuk National University) Corresponding author