논문 상세보기
pp.41-46
In the present study, different expression of protein from Taekwang was revealed by 2-DE, and expressions of protein on each week after flowering was investigated. After analysis of expression of protein, MALDI-TOF was executed to identify expected protein function. Results revealed that there were three patterns of expression of protein during the maturing. The first pattern was that proteins were gradually expressed as up-regulation from 1 week to 6 week. The second pattern was that proteins were expressed gradually from 1 week to 5 week and then it started down-regulation in 6 week. The last pattern was that proteins were gradually as up-regulation from 1 week to 3 week and then down-regulation until 6 week. This phenomenon suggests that young stage has more protein related to correspondence mechanism against disease and growth and then maturing stage has more expression of protein related to storage protein. In MALDI-TOF analysis, p24 oleosin isoform A protein was identified that relates oleosin which is synthetic product in oil body. This protein spot increased gradually until 5 week and then decreased after 5 week. It explained that the protein is active until maturing stage to protect oil in seed and then its activity has gradually degraded. This result may be expected that a protein, related to growth of a seed has increased until maturing and then a seed fills up with a storage protein
식물재료
개화 후 시기별 단백질 추출 및 함량분석
이차원전기영동 및 이미지 분석
질량분석을 이용한 단백질 동정
결과 및 고찰
이차원전기영동을 이용한 개화 후 시기별 단백질 발현 양상 비교
이미지분석을 통한 개화 후 시기별 단백질 발현 비교 분석
질량분석을 이용한 단백질 분석
적 요
인용문헌(REFERENCES)
