Soybean is very useful crop to supply vegetable protein for human. Supply of soybean is increased because it has useful ingredient. Recently, cultivation of soybean in paddy field is increasing due to the increase of rice stockpile in Korea. Hence, in this study, expression of protein was identified regarding different environment for cultivation to investigate the effect of different environment on protein expression. Two-dimensional electrophoresis was performed to investigate the expression of protein using image analysis program to measure degree of protein expression in numerical value. Hannam-kong, Beakcheon-Kong, Hwangkeum-Kong, and Danwon-Kong were used as plant material. 2-DE combined with image analysis revealed that each degree of protein expression of Hannam-Kong and Hwangkeum-Kong in upland field was higher than degree of protein expression in paddy field. However, in case of Beackcheon-Kong, the phenomenon was opposite. In Danwon-kong, the degree of protein expression was not different between up-land field and paddy field. To this end, major protein spots were not different between paddy field and upland field among all cultivars. It could be suggested that protein expression is not severely different by various environment, but different environment affects degree of protein expression.

재료 및 방법
  식물재료
  단백질추출 및 정량분석
  이차원전기영동 수행 및 이미지 분석
 결과 및 고찰
  이차원전기영동을 이용한 재배환경 차이에 따른 단백질 발현 양상 비교 분석
  이미지분석을 이용한 재배환경 차이에 따른 단백질 발현정도 비교 분석
 적 요
 인용문헌(REFERENCES)

• 조성우(국립식량과학원 작물육종과) | Seong-Woo Cho
• 김태선(충북대학교 식물자원학과) | Tae-Sun Kim
• 권수정(충북대학교 식물자원학과) | Soo-Jeong Kwon
• Swapan Kumar Roy(충북대학교 식물자원학과)
• 이철원(충북대학교 식물자원학과) | Chul-Won Lee
• 김홍식(충북대학교 식물자원학과) | Hong-Sig Kim
• 우선희(충북대학교 식물자원학과) | Sun-Hee Woo Corresponding author