In this study, 43 Collected strains of Grifola frondosa were tested in bottle cultivation. In rate of primordia formation, 10 strains were below 50%, 23 strains were 51~79% and 10 strains were over 80%. In pileus color, almost strains were grayish brown and only one strains were light grayish brown. Ivory and white pileus color were showed 2 strains, respectively. The fresh weight of fruit body per bottle of 25 strains were bellow 69g, that of 16 strains were 70~89g and that of 2 strains were over 90g. We selected 9 strains for parent strains of breeding with useful and various genetic characteristics.