Twenty Inter simple sequence repeat (ISSR) and 30 SSR primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. Of them, four ISSR primers, (GA)₈T, (AG)₈YC, (GA)₈C and (CTC)₆and seven SSR markers produced PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. Forty five strains of A. bisporus are genetically clustered into 6 groups, showing coefficient similarity from 0.75 to 0.9 among them. The varieties, Saea, saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.96, whereas, other strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese.

• Kyong-Jin Min(Graduate School of Future Convergence Technology, Hankyong National University)
• Sang-Su Kim(Graduate School of Future Convergence Technology, Hankyong National University)
• A-Min Kwak(Graduate School of Future Convergence Technology, Hankyong National University, JK BioTech. Co. Ltd.)
• Youn-Lee Oh(Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
• Won-Sik Kong(Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
• Hee-Wan Kang(Graduate School of Future Convergence Technology, Hankyong National University, JK BioTech. Co. Ltd.)