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평야지 적응성 향상을 위한 벼흰잎마름병 및 줄무늬잎마름병 저항성 유전자 집적 조생 계통 개발 KCI 등재
Development of Early Maturing Rice Lines with Genes Conferring Resistance to Bacterial Blight and Rice Stripe Virus for Enhancing the Adaptability in Plain Area
pp.118-127
This study was conducted to develop the early maturing rice lines with genes conferring resistance to bacterial blight and rice stripe virus to enhance the adaptability in plain area. Unkwang carrying Xa3 was used as a recurrent parent and SR30075 carrying Xa4+xa5+Xa21+Stvb-i was used as a donor parent. RL1(Resistant Line, BC1F7), RL2, RL3, RL4, and RL5(BC2F6) were bred through bio-assay of K3a race inoculation and phenotypic selection of agronomic traits. The presence of introduced genes was confirmed by testing the resistance levels against bacterial blight and rice stripe virus and then double-checked by using DNA marker. RL1 has all target genes, Xa3+xa5+Xa21+Stvb-i. RL2, RL3, and RL5 have Xa3+Xa21+Stvb-i whereas RL4 has only Xa21. Rice lines carrying Stvb-i showed resistance reaction to rice stripe virus. The combinations of bacterial blight resistant genes(Xa3+xa5+Xa21 and Xa3+Xa21) were found to be promising, as the rice lines carrying these genes enhanced a strong resistant reaction against 16 bacterial blight isolates. Also, the inoculation of K3a race did not alter the brown rice yield, ripened grain ratio and kernel quality of brown rice compared to control. Although RL1 containing all the target resistance genes showed excellent resistance performance, it is not suitable to cultivate in plain area due to instability to lodging, 80% yield level than Unkwang, and low grain quality. RL5 backcrossed twice with Unkwang was found to be a promising line due to its effective resistance gene combination, Xa3+Xa21+Stvb-i and good agronomic traits such as stability to lodging, higher yield and quality compared to Unkwang.
재료 및 방법
시험재료 및 재배방법
벼흰잎마름병 및 줄무늬잎마름병 저항성 검정
저항성 유전자 확인
수량 및 품질 관련 형질 조사
통계 분석
결 과
저항성 계통 육성 및 저항성 유전자 확인
벼흰잎마름병 및 줄무늬잎마름병 저항성 생물검정
육성계통의 농업형질 특성
고 찰
적 요
사 사
REFERENCES
