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형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석 KCI 등재

Selection of β-carotene Enhanced Transgenic Soybean Containing Single-copy Transgene and Analysis of Integration Sites

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  • URLhttps://db.koreascholar.com/Article/Detail/302019
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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

The β-carotene biofortified transgenic soybean was developed recently through Agrobacterium-mediated transformation using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan). GM crops prior to use as food or release into the environment required risk assessments to environment and human health in Korea. Generally, transgenic plants containing a copy of T-DNA were used for stable expression of desirable trait gene in risk assessments. Also, information about integration site of T-DNA can be used to test the hypothesis that the inserted DNA does not trigger production of unintended transgenic proteins, or disrupt plant genes, which may cause the transgenic crop to be harmful. As these reasons, we selected four transgenic soybean lines expressing carotenoid biosynthesis genes with a copy of T-DNA by using Southern blot analysis, and analyzed the integration sites of their T-DNA by using flanking sequence analysis. The results showed that, T-DNA of three transgenic soybean lines (7-1-1-1, 9-1-2, 10-10-1) was inserted within intergenic region of the soybean chromosome, while T-DNA of a transgenic soybean line (10-19-1) located exon region of chromosome 13. This data of integration site and flanking sequences is useful for the biosafety assessment and for the identification of the β-carotene biofortified transgenic soybean.

목차
서 언
 재료 및 방법
  식물재료
  형질전환 작물의 도입유전자 수 결정
  도입유전자 확인을 위한 PCR, RT-PCR 및 immunostrip검정
  도입유전자의 삽입 위치 분석
 결과 및 고찰
  도입유전자의 복제 수 및 발현 분석
  도입유전자의 삽입위치 및 도입유전자의 구조 분석
 적 요
 사 사
 References
저자
  • 친양(농촌진흥청 국립농업과학원) | Yang Qin
  • 권순종(국립식량과학원) | Soon-Jong Kweon
  • 정영수(동아대학교 유전공학과) | Young-Soo Chung
  • 하선화(경희대학교 유전공학과) | Sun-Hwa Ha
  • 신공식(농촌진흥청 국립농업과학원) | Kong-Sik Shin
  • 임명호(농촌진흥청 국립농업과학원) | Myung-Ho Lim
  • 권택윤(농촌진흥청 국립농업과학원) | Taek-Ryoun Kwon
  • 조현석(농촌진흥청 국립농업과학원) | Hyun-Suk Cho
  • 박순기(경북대학교 응용생명과학부) | Soon Ki Park
  • 우희종(농촌진흥청 국립농업과학원) | Hee-Jong Woo