Artificial insemination is a technique to transfer instrumentally sperm from the male into the female’s reproductive system. A key factor among artificial insemination techniques is gathering sperms. Here, a method for collecting sperm of bumblebee male by pressing was firstly developed. This method has stage of separating reprodutive tract of male, separating accessory testis, separating vas deferens, pressing vas deferens with cover-glass and collecting sperm with syringe. The developed method was 2.8 fold higher in rate of collecting sperm than that of existing method. Among 1M NaCl-, Insect ringer solution-, Phosphate buffered saline(PBS)-collecting sperm solutions, PBS was exhibited the best result as 17.2 sperms/cell, which corresponded to 1.6-1.8 fold increased over the result of other collecting sperm solution. Consequently, we think that this method is very important technique to save time for collecting sperm, to keep activity of sperm and to reduce contamination of sperm.

• Hyung Joo Yoon(Department of Agricultural Biology, The National Academy of Agricultural Science, RDA)
• Kyeong Yong Lee(Department of Agricultural Biology, The National Academy of Agricultural Science, RDA)
• Yong Bo Lee(Department of Agricultural Biology, The National Academy of Agricultural Science, RDA)
• Young Hee Cho(Department of Agricultural Biology, The National Academy of Agricultural Science, RDA)