논문 상세보기
Overexpression of the RNA binding gene from Medicago trancatula regulates flowering time
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/302579
FLOWERING TIME CONTROL PROTEIN, FPA gene encode RNA Recognition Motif (RRM) domain protein and plays important roles in flowering time control in Arabidopsis. Floral transition is significant for reproductive products in all flowering plants. However, little is known about the functions of Medicago autonomous pathway gene. We had cloned the FPA gene on Medicago based on the sequence similarity of Arabidopsis FPA sequence. The RT-qPCR analysis of MtFPA expression patterns showed that the MtFPA transcripts accumulated ubiquitously in roots, leaves, stems, flowers, and pods. When fused to the green fluorescence protein, MtPFA-GFP was localized in the nucleus as speckle pattern of protoplast from Arabidopsis. To examine the function of MtFPA, 35S::MtFPA transgenic plants were generated in Arabidopsis late flowering mutant background, fpa-2. Overexpression of MtFPA specifically caused early flowering under long day conditions compared with non-transgenic plants. In MtFPA transgenic lines, AtFLC expression were down-regulated whereas the floral integrators, AtFT and AtSOC1 were up-regulated as compare with control plant. As these results, MtFPA suggest that is a functional ortholog of the Arabidopsis and may play an important role in the regulation of flowering transition in Medicago.
