The oriental melon (C. melo var. makuwa), called ‘Chamoe’ in Korean, is a popular fruit crop cultivated mainly in Asia and a high–market value crop in Korea. To provide a genomic resource as a reference genome for the Cucurbitaceae crop improvement, we performed whole genome sequencing of Korean landrace, Gotgam chamoe. We used Illumina HiSeq2000 sequencing platform to generate 89 Gb (205X) of paired and mate pair sequence reads. The pre-processed reads were de novo assembled resulting in 4,764 scaffolds with a N50 scaffold length of 249kb. This assembly represented 379.8Mb which was 84.7% of the 448Mb of the whole genome. The assembled draft was predicted 26,634 genes of which 80% were predicted by known protein or C. melo unigene homology. Approximately 20% of predicted genes were hypothetical. A total of 1,885 non-coding RNA was detected including rRNA. The transposable elements were accounted for 21% (71.6Mb) of the total assembly. All the marker candidates including SSR, INDEL, SNP were mined and presented. The draft genome will provide a useful platform for genomic research and improvement for Cucurbitacea crops.

• Inkyu Park(Korea Research Institute of Bioscience and Biotechnology (KRIBB), College of Agriculture and Life Science, Chungnam National University)
• Jae-Pil Choi(Korea Research Institute of Bioscience and Biotechnology (KRIBB))
• Jungeun Kim(Korea Research Institute of Bioscience and Biotechnology (KRIBB))
• Jeongyeo Lee(Korea Research Institute of Bioscience and Biotechnology (KRIBB))
• Soohwan Lim(Korea Research Institute of Bioscience and Biotechnology (KRIBB))
• Mi-Ye Lee(Korea Research Institute of Bioscience and Biotechnology (KRIBB), College of Agriculture and Life Science, Chungnam National University)
• Hey-Ran Kim(Korea Research Institute of Bioscience and Biotechnology (KRIBB)) Corresponding Author