In rice (Oryza sativa L.), there is a diversity in flowering time that is strictly genetically regulated by plenty of genes. The floral transition from vegetative to reproductive development is a very important step in the life cycle of a flowering plant. Orthologous genes, which are homologous genes that diverged after a speciation event, generally maintain a similar function in different species. with a McDonald-Kreitman Test (MKT), we examined more than 10,000 orthologous genes between rice (Oryza sativa) and Brachypodium (outgroup), based on different phenotypic groups, to find some fast evolutionary genes of rice flowering time. Three groups with early flowering time (group 1), midium flowering time (group 2) and late flowering time (group 3) were separated and each group was examined for McDonald-Kreitman Test (MKT). Total 70 fast evolutionary genes under a positive selection were found in the three groups, and 14, 42 and 14 genes were specific existed in group 1, 2 and 3, respectively. Annotation of these genes were conducted and the predicted functions were also surveyed. In addition, network analysis of these characterized genes were also investigated to infer related pathways. And also, the association study between the one early flowering factor and the flowering time phenotype was performed and indicated that this gene is significantly correlated with flowering time in rice. These results suggest that using this orthologous based method, we could find some important candidate genes underlying flowering time regulations.