In total, 120 ‘Cheonghcheong/Nagdong’ doubled haploid (CNDH) populations was developed by F1 derived from a crossing whitebacked planthopper (WBPH, Sogatella furcifera) resistance ‘Cheongcheong’ and susceptible ‘Nagdong’ lines. The main objective of this research was to determine the rice resistance optimum screening after infesting by WBPH and identify quantitative trait loci (QTLs) associated with rice resistance in order to provide consistent information for marker-assisted selection (MAS) and develop new varieties. The genetic map with average 9.6 centimorgans (cM) between markers was constructed from 120 CNDH populations using 217 SSR markers. In this study, The result of determine rice with WBPH infestation showed that the rice damage and resistance at 7, 14, and 21 days, were 100%, 76%, and 10% resistance lines of 120 CNDH population. Four QTLs were detected on four regions of the chromosomes 1 and chromosome 8, which contained qWBPH1 and qWBPH8 for resistance rice. The markers were found to be contained in identification the genetic markers RM3482, RM1196, RM3709, RM11694, RM11669, RM17699 and RM264 for marker assisted selection. These markers efficiently were shown to be very useful for MAS in breeding populations of crossing lines associated simple sequence repeat (SSR) marker with WBPH resistance in 120 CNDH populations.