sy-2 (Seychelles-2) is a temperature sensitive natural mutant of Capsicum chinense and native to Seychelles Island in Africa. Previously we showed that sy-2 leaves were irregularly shaped and defective in chlorophyll development at temperatures below 24℃. A segregation test revealed that the sy-2 gene is controlled by a single recessive gene. To identify the sy-2 gene, we performed a map-based cloning approach using a total 600 individual F2 plants derived from crossing sy-2 and the wild type C. chinense ‘No.3341’. Fine-mapping of the locus allowed us to position sy-2 to an approximately 170-kb region flanked by markers IN2-1-1 and SNP-3-7 on chromosome 1. Among the approximately 36 hypothetical genes in this region several candidate genes including: HSP90-like ATPase family proteins, lipid-transfer proteins, calmodulin-domain protein kinases, and zinc finger proteins (ZFPs) were identified. RT-PCR and sequencing of the hypothetical genes are under way to identify sy-2.

• Li Liu(Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University)
• Min-Young Kang(Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University)
• Jin-Ho Kang(Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University)
• Yeong Deuk Jo(Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University)
• Sota Koeda(Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Japan)
• Munetaka Hosokawa(Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Japan)
• Doil Choi(Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University)
• Byoung-Cheorl Kang(Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University) Corresponding Author