In the course of map-based cloning, mutant genes are identified through linkage to specific region on genetic map. Here, we demonstrated gametophytic mutant line, named as AP-28-23, in which mutant gene was mapped on chromosome 2. Based on phenotypic analysis of mature pollen, mutant phenotype of AP-28-23 was classified into three classes, wild-type showing 2-4%, moderate 35-53% and severe type 97-100% on aberrant pollen frequencies, respectively. The severe type is completely sterilized with 100% unfertilized ovules. We also revealed that the transmission was reduced through male gametophyte in the AP-28-23 line. The transmission efficiency (TE) through the male gametophyte is only 0.67%, whereas in the female gametophyte is 89.87%.

• Thi Hoai Thuong Nguyen(School of Applied Biosciences, Kyungpook National University)
• Hyo-Jin Park(School of Applied Biosciences, Kyungpook National University)
• Tien Dung Nguyen(School of Applied Biosciences, Kyungpook National University)
• Sung Aeong Oh(School of Applied Biosciences, Kyungpook National University)
• Soon Ki Park(School of Applied Biosciences, Kyungpook National University) Corresponding Author