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Development of QTL-NIL to Blast Resistance Origined from Korean Weedy Rice
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/302820
Rice blast caused by the fungal pathogen, Magnaporthe oryzae, is a serious disease affecting yield loss and decreasing its quality in rice production. Rice breeders in Korea have developed many japonica varieties showing resistance to blast. However, the blast resistance in most japonica varieties has broken down within a few years after they were released to farmers because of the spread of new virulent races of M. oryzae. There is the most effectiveness to look for novel resistant gene(s) that can express the resistance to broad-spectrum races in diverse environmental conditions. We identified a major QTL, qLB4.1 linked tightly to RM6352 and RM3643 in 52.6 cM region on chromosome 4 related to the resistance for isolate inoculation and nursery test, and neck blast from a Korean weedy rice, Geumleungaengmi33. This QTL explained 26.1∼28.6% and 45.3∼53.1% of total phenotypic variation by the allele of GL33 for isolate inoculation and nursery test, respectively. A line SR30058(52)-1-1 (Suweon545) that containing the QTL qLB4.1 was developed from Ilpum*4/GL33 by marker-assisted backcross method. This line showed resistant reactions to blast nursery test across regions and years, and resistance to neck blast at the hot-spot field in Jecheon. Suweon545 showed also durable resistance of lower 10% of diseased leaf areas (DLAs) in sequential planting method. This line screened by graphical mapping using 136 SSR markers that evenly distributed on 12 chomosomes. Suweon545 contained GL33 alleles of donor parent in a total of 12 loci (8.8%) including QTL region on chromosome 4. In future, Suweon545 would be useful to develop the broad-spectrum resistance variety in japonica rice breeding program.
