Many spectral imaging technologies are available to nondestructive means to assess plant status including abiotic and biotic stress conditions. In recent years, ARS has developed various sensing and instrumentation technologies for agricultural applications. These include hyperspectral imaging for visible/near-infrared (NIR) reflectance and fluorescence imaging, and multispectral laser-induced fluorescence imaging. Hyperspectral imagery is a fusion of imaging and traditional spectroscopy. We recently expanded the hyperspectral capabilities to include rapid macro-scale Raman chemical imaging. The current state of the art of imaging technologies and their potential applications for characterization of the plant status are presented.