Zein/Poria cocos extract nanofibers were prepared based on the electrospinning of aqueous solutions with different Poria cocos extract concentrations (0, 5, 10, and 15 wt.%). The electrospinning parameters, including the polymer contents, voltage, and tip-to-collector distance (TCD), were optimized for the fabrication process. The resulting electrospun materials were all characterized using field-emission scanningelectron microscopy (FE-SEM). The diameters of the electrospun zein nanofibers were found to increase when increasing the zein concentration.