Hip muscle activation and strengthening exercise programs are often used to prevent and treat various lower extremity injuries. Common exercise programs include squat exercises. The purposes of this study were to investigate gluteus medius (GMED) and tensor fasciae latae (TFL) muscle activity, and to assess the GMED/TFL ratio during squat exercises involving different isometric hip contraction conditions. Different types of isometric hip contraction were standard squat without hip contraction, squats with isometric hip adduction, and squats with isometric hip abduction. Twenty (10 males and 10 females) healthy subjects (23.7±2.8 years old) were recruited. Subjects performed the squat exercises with the back supported by a wall and knees flexed to 60°. Surface electromyography (EMG) was used to measure GMED and TFL activity. One-way repeated analysis of variance was used to compare GMED and TFL muscle activity and the GMED/TFL ratio. GMED and TFL EMG activity was significantly higher during squats with isometric hip adduction and abduction compared with the standard squat without hip contraction (p<.05). Between the isometric hip adduction and abduction contraction conditions, only the TFL EMG activity was significantly higher during squats with isometric hip adduction than isometric hip abduction (p<.05). The GMED/TFL ratio was significantly higher during squats with isometric hip adduction than isometric hip abduction (p<.05). Squats with isometric hip adduction and abduction improved GMED and TFL muscle activity. Furthermore, the GMED/TFL ratio was higher during isometric hip adduction than isometric hip abduction. Our data indicate that squat exercises involving isometric hip adduction enhance GMED muscle activity.