Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature(25±1oC) unlikeA. bisporus is cultivate at 16±2oC. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at hightemperature (25±1oC). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we madeheterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(singlespores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated onCDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different mediacondition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon thatcross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.

서 론
 재료 및 방법
  모본 선발 및 단포자 분리
  단포자 분리
  교잡 및 교잡 확인
 결과 및 고찰
  모본 선발 및 단포자 분리
  동핵균주 분리 및 교잡주확인을 위한 RAPD분석
 적 요
 감사의 말씀
 References

• 오연이(고려대학교 생명공학과 대학원 생명공학과&BK21플러스) | Youn-Lee Oh
• 장갑열(농촌진흥청 국립원예특작과학원 버섯과) | Kab-Yeul Jang
• 공원식(농촌진흥청 국립원예특작과학원 버섯과) | Won-Sik Kong
• 신평균(농촌진흥청 국립원예특작과학원 버섯과) | yung-Gyun Shin
• 김은선(농촌진흥청 국립원예특작과학원 버섯과) | Eun-Sun Kim
• 오민지(농촌진흥청 국립원예특작과학원 버섯과) | Min ji Oh
• 최인걸(고려대학교 생명공학과 대학원 생명공학과&BK21플러스) | In-Geol Choi Corresponding author