This study was performed in order to analyze the fibrinolytic, thrombin inhibitory, anti-oxidative, acetylcholinesteraseinhibitory, and immuno-enhancing activities of the water extract and solvent fractions isolated from Grifola frondosa. Fibrinolyticactivity was analyzed using the fibrin plate method, and thrombin inhibitory activity was assayed using the substrate H-D-Phe-pip-arg-pna. Anti-oxidative activity was estimated using the DPPH assay, and AChE inhibitory activity was measured using thespectrophotometric method. Immuno-enhancing activity was examined using the nitric oxide (NO) production in RAW 264.7macrophage cells. Cell viability was determined using the MTS assay. Fibrinolytic activities were the highest in water extract (1.55plasmin units/mL) followed by water fraction (0.85 plasmin units/mL). The thrombin inhibitory activities of the water and ethylacetate fractions were determined to be 76.43% and 72.59%, respectively. The acetylcholinesterase inhibitory activities ofchloroform and hexane fractions exhibited values of 95.14% and 94.74%, respectively. The butanol fraction showed the highestanti-oxidative activity at 94.47%. Anti-proliferating activity against Raw 264.7 cells showed no cytotoxicity. The production of NOin Raw 264.7 cells increased up to 2-fold by adding the water fraction compared to the untreated control. These results suggestthat Grifola frondosa may serve as a useful functional food for the enhancement of immune function and the prevention andtherapy of cardiovascular diseases.

서 론
 재료 및 방법
  실험재료
  세포배양
  물 추출물과 유기용매 분획물 조제
  혈전용해활성 측정
  트롬빈 저해활성 측정
  Acetylcholinesterase(AchE) 저해 활성
  전자공여능 측정
  세포독성 측정
  NO 생성 저해 효과 측정
  통계 분석
 결과 및 고찰
  용매별 분획물의 수율
  혈전용해활성
  트롬빈 저해활성
  Acetylcholinesterase 저해 활성
  항산화효과
  세포독성 효과
  Nitric Oxide(NO) 생성에 미치는 효과
 적 요
 References

• 김은정(상지대학교 임상병리학과) | Department of Biomedical Laboratory Science, Sangji University, Wonju 220-702, Korea
• 김준호(상지대학교 정밀화학신소재학과) | Jun-Ho Kim Corresponding author