Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.

서 론
 재료 및 방법
  1. 정액 채취
  2. 동결정액 제조
  3. 정자 운동학적 특성 분석
  4. Genomic DNA 분리 및 유전자형 분석
  5. 통계 분석
 결 과
 고 찰
 REFERENCES

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• 우제석(농촌진흥청 국립축산과학원) | Jae-Seok Woo
• 이미진(농촌진흥청 국립축산과학원) | Mi-Jin Lee
• 고준호(농촌진흥청 국립축산과학원) | Jun-Ho Ko
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