Spermiation was stimulated in the mature grass puffer, Takifugu niphobles, with an injection of human chorionic gonadotropin (HCG) or carp pituitary extract (CPE). Spermatocrit and sperm density were reduced, but milt production was increased in both the HCG and CPE treatment groups relative to those in the control group (P < 0.05). These results should be useful for increasing the fertilization efficiency in grass puffer breeding programs.

INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES

• In Bon Goo(Inland Aquaculture Research Center, National Fisheries Research & Development Institute) Corresponding author
• In-Seok Park(Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
• Hyun Woo Gil(Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
• Jae Hyun Im(Inland Aquaculture Research Center, National Fisheries Research & Development Institute)