Red spotted grouper, Epinephelus akaara is a popular aquaculture species in many Asian countries. This species is a protogynous hermaphrodite that first differentiates into female and changes to male later. Due to this reproductive characteristic, stable supply of male and female gametes is a key to the success of seed production in this species. Thus, understanding early gonadal differentiation is required to develop effective sex control techniques. Red spotted grouper were reared in indoor tanks and sampled every 5 days from 40 days post-hatch (DPH) to 130 DPH. Changes of gonadal tissues were examined and analyzed by means of histology. A pair of gonadal primordium has already existed underneath the kidney in the posterior part of the body cavity at 38 DPH when this study began. Gonadal primordia of 38, 40 DPH consisted of germ cells surrounded by a few somatic cells. The blood vessel was observed in the gonadal primordium at 45 DPH. The number of somatic cells and size of gonadal primordium increased age-dependently up to 60 DPH. Formation of ovarian cavity was obvious by two protuberant aggregations of somatic cells at 65 DPH. Completed ovarian cavity and oogonia were first observed in the gonad of one fish sample at 105 DPH. Based on these histological observations, it can be suggested that induction of primary male differentiation could be more successfully applied at around 60 DPH in this species.

INTRODUCTION
MATERIAL & METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENT
REFERENCES

• Hyun Kyu Kim(Dept. of Aquatic Life Medical Science, Sunmoon University)
• Jung-Hyun Kim(Dept. of Aquatic Life Medical Science, Sunmoon University)
• Woo Sik Kim(Dept. of Aquatic Life Medical Science, Sunmoon University)
• Hea Ja Baek(Dept. of Marine Biology, Pukyong National University)
• Joon Yeong Kwon(Dept. of Aquatic Life Medical Science, Sunmoon University) Corresponding author