Impact factor for used in the load carrying capacity evaluation of bridges is varied depending on vehicle speed and bridge frequencies. So, it is hard to define its peak value since in the field test the truck speeds applied cannot cover all possible vehicle speeds and the speed per each vehicle loading test cannot remain constant consistently. Furthermore, the target bridges should be closed during field test, which leads to an inconvenient traffic flow. In this paper, a displacement-based response spectrum of bridges is considered to define the peak impact factor without conducting the standard vehicle loading test, while using a bridge operational traffic condition.