논문 상세보기
사육환경에 따 른 이매패류 (Crassostrea gigas, Mytilus galloprovincialis)의 외부형질 성장과 Heat Shock Protein 70 유전자 발 현 KCI 등재
Expression of the Heat Shock Protein 70 Gene and External Developmental Traits of Two Bivalvia Species, Crassostrea gigas and Mytilus galloprovincialis, under Aquaculture Environments.
pp.22-30
Environmental changes exert harmful effects on organisms inhabiting coastal regions. These changes are also associated with reduced production in aquaculture farms. In this study, we investigated internal and external responses of two Bivalvia species (Crassostrea gigas and Mytilus galloprovincialis) in Gamak Bay under stressful environmental conditions in aquaculture farms. We investigated external responses such as weight, size, and environment exposure time, and analyzed the expression of the HSP70 gene. C. gigas HSP70 gene expression level was significantly high in the C3 aquaculture farm site, but the weight and size of C. gigas were high in the C2 aquaculture farm site. The response of C. gigas HSP70 mRNA was associated with the environmental exposure time in each aquaculture farm. Expression of M. galloprovincialis HSP70 gene was found to be significantly higher in the M2 aquaculture farm site than in the M1 site, whereas the weight of M. galloprovincialis was observed to be higher in the M1 site. The size and environmental exposure time of M. galloprovincialis were similar between M1 and M2 sites. In addition, HSP70 sequences of C. gigas and M. galloprovincialis showed high similarity with that of another marine species. According to our results, there were differences in internal responses following environmental stress in aquaculture farms, with respect to HSP70 gene expression. The results suggest that the HSP70 gene is a useful molecular indicator for monitoring stress responses in Bivalvia species in the field.
재료 및 방 법
1. 실험 생물
2. RNA 추출 및 cDNA 합성
3. Real-time RT-PCR을 통한 유전자 증폭
4. HSP 70 유전자의 계통진화적 분석
5. 통계적 자료분석
결 과
1. C. gigas와 M. galloprovincialis의 HSP 70 계통진화분석
2. 양식장 사육기간에 따른 C. gigas 체중량, 각장과 각고
3. 양식장 사육기간에 따른 M. galloprovincialis 체중량,각장과 각고
4. C. gigas와 M. galloprovincialis의 HSP 70 유전자발현
고 찰
적 요
사 사
References
