LED lighting has an advantage of adjusting color temperature. This change of color temperature may derive change in behavioral and physiological responses of the visual perception for indoor environments. This research examined the changes of behavioral and physiological responses caused by the color temperature. The environment was configured that the indoor temperature was 20 degrees centigrade or less as the perceived uncomfortable environment in winter. Then, the comfortable sensation vote (CSV) and the results of 3-back working memory test were measured as behavioral responses. In addition, the Electrodermal Activity (EDA) and Electrocardiogram (ECG) were measured as the responses of autonomics nervous system (ANS) in the three conditions of color temperature (red: 3862K, white: 5052K, blue: 11,460K). As a result, behavioral responses were not significant by the condition of color temperature, but the tendency of occupants’ physiological relaxation appeared in the blue color temperature condition compared with the white color temperature condition. Although the color temperature of LED lighting might be a small factor in terms of the characteristics of indoor environment, it suggests that the color temperature could have an impact on the physiological changes in the parasympathetic nervous system.