Hyperlipidemia has been ranked as one of the greatest risk factors contributing to the prevalence and severity of coronary heart diseases. The pharmacological actions of tangerine (Citrus unshiu) peel include the facilitation of fat digestive enzymes. Also, guarana (Paullinia cupana) has been used for stimulants and tonics over a long period. In this study, we aimed to optimize the mixed ratio of organic tangerine peel and guarana extracts to suppress fat accumulation. To determine the optimized the mixed ratio of tangerine (Citrus unshiu) peel extract (C) and guarana (Paullinia cupana) extract (P) on adipogenesis, maturing preadipocytes were incubated during the 8-day induction period with various ratio of the mixed extracts groups like as Vehicle (DMEM 200 μl/ml), Con (MDI DMEM 200 μl/ml), C10 (MDI DMEM 180μl/ml+C 20 μl/ml), C9:P1 (MDI DMEM 180 μl/ml+C 18 μl/ml+P 2 μl/ml), C5:P5 (MDI DMEM 180 μl/ml+C 10 μl/ml+P 10 μl/ ml), P10 (MDI DMEM 180 μl/ml+P 20 μl/ml). Thereafter, the adipocytes were stained with Oil-Red-O and analyzed for lipid contents. As the results, organic tangerine peel and guarana extracts were revealed to reduce fat accumulation in 3T3-L1 cells. The fat accumulation significantly decreased in C5:P5 group, which is equally mixed with organic tangerine peel and guarana extracts, as compared to other groups. Based on these results, we found the optimized ratio with organic tangerine peel and guarana extracts to suppress fat accumulation. We suggest that this optimized organic tangerine peel and guarana complex might reduce effectively the serum lipid components and improve the lipid metabolism in hyperlipidemic patient.

Introduction
 Materials and Methods
  추출물 시료의 제조
  진피의 naringin 함량 분석
  과라나 카페인 함량 분석
  세포배양 및 시약 처리
  Oil red O stain
  통계처리
 Results
  진피 열수추출물의 naringin 함량
  과라나 열수추출물의 caffeine 함량
  3T3-L1 지방세포의 분화에 미치는 영향
 Discussion
 References

• Hyun-A Lee(Center for Animal Resources Development, Animal Disease Research Unit, Wonkwang University, Iksan, 54538, Korea)
• Sunhwa Hong(Center for Animal Resources Development, Animal Disease Research Unit, Wonkwang University, Iksan, 54538, Korea)
• Yungho Chung(Department of Companion Animal and Animal Resources Science, Joongbu University, Geumsan, 32713, Korea)
• Okjin Kim(Center for Animal Resources Development, Animal Disease Research Unit, Wonkwang University, Iksan, 54538, Korea)